3 (2001)ĪLTEX - Alternatives to animal experimentation: Vol. ĪLTEX - Alternatives to animal experimentation: Vol. Screening of budesonide nanoformulations for treatment of inflammatory bowel disease in an inflamed 3D cell-culture modelĪLTEX - Alternatives to animal experimentation: Vol. Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicologyĪLTEX - Alternatives to animal experimentation: Vol. Human alveolar epithelial cells expressing tight junctions to model the air-blood barrierĪLTEX - Alternatives to animal experimentation: Vol. Our results suggest that reconstructed skin equivalents based on human keratinocytes have potential as a pharmaceutical test system to study dermal drug transport from topical formulations. Finally the permeability of the reconstructed skin was compared to human epidermis, and a five times higher flux value was found for the skin equivalent model. Two different batches of the skin equivalent showed no statistically significant difference. Flux values were calculated and the permeation across the skin equivalent from the solution was noted to be almost forty times higher than from the ointment. It was shown that the model forms a barrier towards diffusion by comparing the permeation across the tissue-free inserts to the equivalents. HPLC was used for the analysis of drug content. The drug was applied in two pharmaceutical formulations to the intact surface of the skin disk: dissolved in wool alcohol ointment (0.1125 %), and dissolved in Soerensen phosphate buffer pH 7.4 (0.1125% solution). Permeation studies with the Franz diffusion cell were undertaken to evaluate the model for the establishment of a new in vitro method to study the percutaneous absorption of different dosage forms. One such equivalent is EpiDermTM (EPI-606, MatTek corporation, Ashland Massachusetts) which was investigated using the lipophilic model drug flufenamic acid. Since nowadays reconstructed skin equivalents are commercially available, we examined these cultures for their suitability as a percutaneous absorption model for different pharmaceutical formulations. The limited supply and the relatively high donor variability stimulated many research groups to use animal skin as a substitute for human skin. Excised human skin has so far been considered to be one of the most suitable in vitro methods to evaluate the penetration of dermatologically applied substances.
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